Castleport Test Prep

Free VTNE Practice Test: 50 Questions With Answers Explained

These 50 original, unofficial practice questions cover all 10 VTNE content domains in roughly the same proportions as the real exam. Choose your answer, then check the explanation under each question — no signup, and keep a calculator handy for questions 4, 16, 28 and 48.

Practice questions

Question 1 of 50 · Animal Care and Nursing

What is a normal capillary refill time (CRT) in a healthy dog or cat?
Check answer for question 1

Answer: C. 1–2 seconds

Merck's triage parameters list a normal CRT of 1–2 seconds, reflecting normal peripheral perfusion.

Why not the others: A — faster than the normal range listed. B and D — longer than normal; a prolonged CRT is a perfusion finding to report to the veterinarian.

Source: Merck (MSD) Veterinary Manual, table "Parameters to Evaluate During Triage"

Question 2 of 50 · Pharmacy and Pharmacology

A diabetic cat's insulin is labeled 40 IU/mL (U-40). Which syringe should be used to draw up each dose?
Check answer for question 2

Answer: A. A U-40 insulin syringe

U-40 veterinary insulins (for example ProZinc and Vetsulin) are 40 IU/mL, and their labels state that using a syringe other than a U-40 syringe results in incorrect dosing. Syringe markings are calibrated to one concentration.

Why not the others: B — a U-100 syringe's markings assume 100 IU/mL: drawing U-40 insulin to the "10" mark (0.1 mL) delivers only 4 IU. C — converting by eye from mL markings invites dosing errors and isn't what the label directs. D — speed of drawing doesn't fix a calibration mismatch.

Source: ProZinc prescribing information, Package Insert for Cats, “Description” and “Dosage and Administration” (revised 08/2019; DailyMed updated 22 Jan 2025); Vetsulin prescribing information, “Description,” “Dosage and Administration — Vials,” and “How Supplied” (revised 07/2026; DailyMed updated 23 Sep 2026)

Question 3 of 50 · Surgical Nursing

What is the minimum exposure time for wrapped instruments in a gravity-displacement steam sterilizer at 121 °C (250 °F)?
Check answer for question 3

Answer: D. 30 minutes

CDC's table of minimum steam cycle times lists 30 minutes at 121 °C for wrapped instruments in a gravity-displacement sterilizer.

Why not the others: A — 4 minutes is the minimum for wrapped instruments in a dynamic-air-removal (prevacuum) cycle at 132 °C. B — not a listed minimum for this cycle. C — 15 minutes is the gravity-displacement time at the higher 132 °C setting.

Source: CDC, "Steam Sterilization Cycle Times," Table 7 (page updated 20 Dec 2023)

Question 4 of 50 · Anesthesia · Calculation

A 4-kg cat is maintained on a non-rebreathing circuit. Using an oxygen flow of 200–400 mL/kg/min, what flow range is appropriate?
Check answer for question 4

Answer: B. 800–1,600 mL/min

4 kg × 200 mL/kg/min = 800 mL/min; 4 kg × 400 mL/kg/min = 1,600 mL/min (0.8–1.6 L/min). AAHA's anesthesia guidelines describe non-rebreathing circuits for patients under about 3–5 kg, with oxygen flow around 200–400 mL/kg/min.

Why not the others: A — that's 20–40 mL/kg/min, the rebreathing-circuit range, too low for a non-rebreathing system. C — a decimal error (ten times too high). D — 500 mL/min is AAHA's minimum flow for rebreathing circuits, not a non-rebreathing rate.

Source: Grubb T et al., 2020 AAHA Anesthesia and Monitoring Guidelines for Dogs and Cats, Phase 2 Step 2b, PDF pp.7–8

Question 5 of 50 · Laboratory Procedures

Which fecal flotation approach gives the best chance of detecting parasite eggs?
Check answer for question 5

Answer: A. Centrifugal flotation using a solution with a specific gravity of 1.18–1.27

CAPC states centrifugal flotation is consistently more sensitive than simple flotation, and flotation solutions should have a specific gravity of about 1.18–1.27 (Sheather's sugar solution is 1.27; sodium nitrate 1.18–1.20).

Why not the others: B — the right solution, but simple flotation recovers fewer eggs than centrifugation. C and D — solutions below the 1.18–1.27 range (tap water is about 1.00) are too light to float many eggs.

Source: Companion Animal Parasite Council, “Microscopic Fecal Exam Procedures,” sections “Why Fecal Centrifugation is Better” and “Fecal Flotation Basics”

Question 6 of 50 · Animal Care and Nursing

A technician needs to pick up a pet rabbit for a physical exam. Which handling technique best protects the rabbit?
Check answer for question 6

Answer: C. Support the hind legs and tuck the hindquarters into the crook of the arm

UC Davis stresses supporting a rabbit's back legs whenever it is picked up, with the rear end tucked into the crook of the arm. Rabbits can injure their backs if they struggle vigorously or jump from a height, so the hindquarters need support.

Why not the others: A and D — neither supports the back legs, which is the key protection. B — unsupported hind legs let the rabbit struggle, the situation that risks a back injury.

Source: UC Davis School of Veterinary Medicine, "Rabbit Care," section "Handling"

Question 7 of 50 · Dentistry

During a dental exam under anesthesia, the probe slips 6 mm into the gingival sulcus of a midsized dog's canine tooth. How should this be interpreted?
Check answer for question 7

Answer: B. Deeper than the usual 1–3 mm reference range for dogs — chart it and report it

Merck gives normal probing depth as about 1–3 mm in dogs and 0.5–1 mm in cats. A 6-mm reading exceeds that and belongs on the dental chart for the veterinarian.

Why not the others: A and D — 6 mm exceeds the expected range for this midsized dog; canine root length alone does not make that reading normal. C — cats' normal depth (0.5–1 mm) is shallower than dogs', so the species don't share one value.

Source: Merck Veterinary Manual, “Periodontal Disease in Small Animals,” “Periodontal Probing” (updated Apr 2025); 2019 AAHA Dental Care Guidelines for Dogs and Cats, “Essential steps of dental cleaning & therapy,” step 5 “Periodontal probing”

Question 8 of 50 · Emergency Medicine/Critical Care

A cat needs its first-ever blood transfusion. What should happen before blood is given?
Check answer for question 8

Answer: D. Blood type the cat (A/B/AB); a major crossmatch is also strongly recommended

Cats can have strong naturally occurring alloantibodies — type B cats carry high-titer anti-A antibodies — so Merck calls AB typing essential before transfusion and strongly recommends a major crossmatch even before a cat's first transfusion.

Why not the others: A and B — naturally occurring antibodies mean a first transfusion can react. C — type A blood given to a type B cat meets strong anti-A antibodies.

Source: Merck Veterinary Manual, “Blood Transfusions in Dogs and Cats,” feline blood typing and transfusion compatibility (updated Mar 2026); Merck (MSD) Veterinary Manual, “Crossmatching in Dogs and Cats” (reviewed/updated Nov 2024); 2021 ISFM Consensus Guidelines on the Collection and Administration of Blood and Blood Products in Cats, “Alloantibodies” and “Cross-matching”

Question 9 of 50 · Pain Management/Analgesia

A dog is excessively sedated after receiving an opioid, and the veterinarian wants the opioid's effects reversed. Which drug does this?
Check answer for question 9

Answer: A. Naloxone

Naloxone is an opioid antagonist used to reverse opioid effects; the 2024 RECOVER guidelines list it as a reversal agent after opioid exposure.

Why not the others: B — a phenothiazine tranquilizer; it adds sedation rather than reversing it. C — an NSAID analgesic. D — a fluoroquinolone antibiotic.

Source: Burkitt-Creedon JM et al., 2024 RECOVER Guidelines: Updated treatment recommendations for CPR in dogs and cats, Tables 1–2; AAHA, Ralph Harvey, “Crisis Management: How to Handle Emergencies and Complications,” “Human Error,” PDF p.2 (2011); Acepromazine maleate tablet manufacturer label, “Mode of Action” and “Precautions” (DailyMed); Meloxidyl (meloxicam) oral suspension manufacturer label, “Description” and “Indications” (DailyMed); EnroMed 100 (enrofloxacin) manufacturer label, “Description” and “Microbiology” (DailyMed)

Question 10 of 50 · Diagnostic Imaging

In a ventrodorsal (VD) radiograph, the x-ray beam:
Check answer for question 10

Answer: C. Enters the ventral surface and exits the dorsal surface

Radiographic projections are named for where the beam enters and then exits. Ventrodorsal = in through the ventral surface, out through the dorsal surface.

Why not the others: A — that describes a dorsoventral (DV) view. B — that describes a lateral projection. D — the name follows the beam's path, not which surface touches the table.

Source: Introduction to Veterinary Terminology (open textbook), “2.4 Directional and Movement Terms,” “Clinical Insight”

Question 11 of 50 · Animal Care and Nursing

A kennel run held a puppy with confirmed parvovirus. After cleaning its hard, nonporous surfaces, which disinfectant is the best choice?
Check answer for question 11

Answer: B. A sodium hypochlorite product labeled effective against canine parvovirus, used at its labeled dilution and full wet contact time

Canine parvovirus is non-enveloped and resists many disinfectants. Merck lists 5% sodium hypochlorite diluted 1:32 as one virucidal example. For the product in use, follow its label directions for canine parvovirus for dilution and the full wet contact time; bleach stock strengths and product directions vary.

Why not the others: A — this product has no demonstrated efficacy against canine parvovirus; efficacy should not be inferred from chemical class alone. C — AAHA notes alcohols are likely not the best choice for non-enveloped viruses. D — cleaning removes organic debris, but it isn't the virucidal step.

Source: Merck Veterinary Manual, “Canine Parvovirus Infection (Parvoviral Enteritis in Dogs),” “Etiology” and “Prevention and Control” (reviewed/updated Jun 2025); 2018 AAHA Infection Control, Prevention, and Biosecurity Guidelines, “Overview of ICPB Procedures,” “Cleaning and Disinfection”; AAHA, “Infection control, prevention, and biosecurity: Making a plan,” “Cleaning and disinfection” (2 Oct 2024)

Question 12 of 50 · Pharmacy and Pharmacology

Which statement about the MDR1 (ABCB1) gene mutation in dogs is accurate?
Check answer for question 12

Answer: A. Loperamide should be avoided in dogs that carry the mutation.

Cornell's MDR1 resource says loperamide should be avoided entirely in dogs with the mutation because of their risk of drug toxicity.

Why not the others: B — Cornell lists the mutation in Australian shepherds and Shetland sheepdogs as well as collies. C — heartworm preventives remain safe at low, FDA-approved label doses; higher ivermectin doses can cause toxicity. D — genetic testing identifies whether a dog carries the variant.

Source: Cornell University College of Veterinary Medicine, “Drug sensitivity: MDR1,” “Breed predisposition,” “Drugs of concern,” “Diagnosis,” and “Genetics”

Question 13 of 50 · Surgical Nursing

Hemostats are being placed into an instrument pack for steam sterilization. How should they be positioned?
Check answer for question 13

Answer: D. Opened (unlocked) so steam can contact all surfaces

CDC's sterilizing-practices guidance, citing AAMI, says hinged instruments should be opened before sterilization so the sterilant reaches all surfaces.

Why not the others: A, B, C — a closed position can prevent steam from directly contacting all instrument surfaces.

Source: CDC, "Sterilizing Practices," section "Packaging" (updated 28 Nov 2023)

Question 14 of 50 · Anesthesia

During maintenance anesthesia, a spontaneously breathing patient is on a rebreathing circuit with fresh gas flowing. The adjustable pressure-limiting (APL, "pop-off") valve was left fully closed. What is the main risk?
Check answer for question 14

Answer: C. Pressure buildup in the circuit and lungs, causing pulmonary barotrauma

With the valve closed, fresh gas has no exit, so pressure builds in the breathing system and the patient's lungs — leading to barotrauma and, at the extreme, pneumothorax. AAHA instructs reopening the APL valve after a successful machine leak test; temporary closure can also be used during monitored manual ventilation.

Why not the others: A, B, D — none is the immediate danger of a closed valve; rising circuit pressure is.

Source: Veterinary Anesthesia & Analgesia Support Group, “Avoiding pop-off valve related fatalities”; 2020 AAHA Anesthesia and Monitoring Guidelines, Phase 2 Step 2c, PDF p.8 (leak test)

Question 15 of 50 · Communication and Veterinary Professional Support Services

After euthanasia, which single finding confirms death on its own?
Check answer for question 15

Answer: B. Rigor mortis

The AVMA euthanasia guidelines say a combination of criteria is most reliable for confirming death, and that none of the signs alone confirms death except rigor mortis.

Why not the others: A, C, D — each is one of the AVMA's confirming criteria, but on its own none confirms death.

Source: AVMA Guidelines for the Euthanasia of Animals: 2020 Edition, Section I7 “Confirmation of Death,” pp.16–17

Question 16 of 50 · Animal Care and Nursing · Calculation

Using RER = 70 × (body weight in kg)^0.75, what is the resting energy requirement of a 10-kg dog?
Check answer for question 16

Answer: A. About 394 kcal/day

10^0.75 ≈ 5.62; 70 × 5.62 ≈ 394 kcal/day. Use the y^x (power) key on a scientific calculator.

Why not the others: B — multiplies 70 × 10 and skips the exponent. C — uses 100 instead of 70 as the multiplier. D — a decimal-place slip.

Source: 2021 AAHA Nutrition and Weight Management Guidelines, Box 1 “Energy Requirement Calculations,” PDF p.1

Question 17 of 50 · Laboratory Procedures

A canine heartworm antigen test primarily detects antigen from:
Check answer for question 17

Answer: D. Adult female worms

The American Heartworm Society explains that the antigen detected comes mainly from the reproductive tract of adult female Dirofilaria immitis. Current heartworm antigen tests do not reliably detect infections made up only of male worms.

Why not the others: A and B — antigen tests aren't designed to detect immature stages. C — male-only infections are the ones antigen tests can miss.

Source: American Heartworm Society, Canine Guidelines (revised 2024; file dated 19 Jun 2025), “Antigen Tests,” printed pp.14–15 (PDF pp.16–17)

Question 18 of 50 · Surgical Nursing

An enterotomy is performed under controlled conditions with no unusual spillage. No acute inflammation, pre-existing infection or perforation, or break in sterile technique is encountered. How is the surgical wound classified?
Check answer for question 18

Answer: B. Clean-contaminated

CDC's surgical wound classification puts operations that enter the alimentary, respiratory, genital, or urinary tract under controlled conditions, without unusual contamination, in Class II: clean-contaminated.

Why not the others: A — clean wounds don't enter those tracts. C — gross spillage, a major break in sterile technique, or acute nonpurulent inflammation would support a contaminated classification. D — existing infection or perforated viscera would support a dirty/infected classification.

Source: Mangram AJ et al., CDC Guideline for Prevention of Surgical Site Infection, 1999, Table 7 "Surgical Wound Classification," p.109

Question 19 of 50 · Anesthesia

Right after intubation, the capnograph shows an ETCO₂ near zero with no sustained waveform. The dog is breathing and has a palpable pulse, and the capnograph and sampling connection have been checked and are functioning correctly. What is the most likely cause?
Check answer for question 19

Answer: C. The tube is in the esophagus

AAHA uses a definitive ETCO₂ tracing to confirm tube placement. The esophagus holds very little CO₂, so an esophageal tube reads very low to zero. Tell the veterinarian and recheck placement. An absent waveform is not diagnostic by itself; other patient and equipment causes of signal loss must also be considered.

Why not the others: A — hyperthermia can alter ventilation but does not explain the absent sustained waveform in this setting. B — with functioning monitoring and adequate ventilation and perfusion, a correctly placed tube should produce a sustained CO₂ waveform. D — hypercapnia is increased carbon dioxide, not an explanation for the absent exhaled CO₂ signal.

Source: 2020 AAHA Anesthesia and Monitoring Guidelines, Step 4c, PDF p.17; Step 2 equipment checks, PDF p.6; Shippy S, “Capnography in Anesthesia,” Today’s Veterinary Practice, Jul/Aug 2024, pp.74–76: tube placement, loss of waveform, and decreased ETCO₂

Question 20 of 50 · Pharmacy and Pharmacology

The U.S. label for enrofloxacin warns against exceeding 5 mg/kg per day in cats. What adverse effect is the warning about?
Check answer for question 20

Answer: A. Retinal toxicity, including blindness

The FDA-approved Baytril label states not to exceed 5 mg/kg/day in cats because rare retinal toxicity has been reported; in safety studies, higher doses caused retinal changes and blindness.

Why not the others: B, C, D — none is the cat-specific warning on the label.

Source: FDA Freedom of Information Summary, NADA 140-441 (Baytril), §III.B label warnings and §III.A.5

Question 21 of 50 · Animal Care and Nursing

While auscultating a dog, you count an irregular heart rate of 180 beats/min, but you palpate only 140 femoral pulses/min at the same time. What does this finding suggest?
Check answer for question 21

Answer: D. A pulse deficit — suggestive of an arrhythmia; report it, since an ECG is indicated

Fewer pulses than heartbeats is a pulse deficit. Merck notes that tachycardia with irregularity or pulse deficits usually means an arrhythmia, and an ECG is indicated.

Why not the others: A and B — a pulse deficit is an abnormal finding, not something to dismiss. C — this finding does not by itself establish dehydration or justify fluid treatment; report it promptly for assessment.

Source: Merck (MSD) Veterinary Manual, "Parameters to Evaluate During Triage"; University of Guelph, "Understanding Normal and Abnormal Heart Rhythm"

Question 22 of 50 · Pain Management/Analgesia

U.S. veterinary meloxicam labels carry a boxed warning about cats. What does it warn against?
Check answer for question 22

Answer: B. Acute renal failure and death with repeated use

The boxed warning states that repeated use of meloxicam in cats has been associated with acute renal failure and death, and that additional injectable or oral doses should not be given to cats. It appears on all brand and generic veterinary meloxicam labels.

Why not the others: A and D — not what the boxed warning describes. C — retinal toxicity is the cat warning for enrofloxacin, not meloxicam.

Source: FDA, “Information About the Boxed Warning on Meloxicam Labels Regarding Safety Risks in Cats” (content current as of 26 Nov 2024); FDA Freedom of Information Summary, NADA 140-441 (Baytril), §III.B label warnings and §III.A.5

Question 23 of 50 · Diagnostic Imaging

Under the cited University of Pennsylvania veterinary radiation-safety policy, where should a technician wear the routine whole-body film badge when wearing a lead apron and thyroid shield during radiography?
Check answer for question 23

Answer: C. At collar level, outside the lead apron and thyroid shield

The University of Pennsylvania veterinary radiation-safety policy directs staff to wear the film badge at collar level above protective clothing. This records exposure at that unshielded location; badge placement must follow the facility's monitoring program.

Why not the others: A — under the apron, the badge would underestimate the dose to unshielded areas. B — a wrist placement is not the whole-body badge position these rules specify. D — a wall badge doesn't measure the person's dose.

Source: University of Pennsylvania EHRS, Veterinary Energized Equipment User’s Guide (2012 edition), §V.B “Dosimeter Placement,” PDF p.4

Question 24 of 50 · Laboratory Procedures

After a microhematocrit tube is centrifuged, what does the buffy coat contain?
Check answer for question 24

Answer: A. White blood cells and platelets

eClinpath describes the buffy coat — the thin layer between the packed red cells and the plasma — as containing white blood cells and platelets.

Why not the others: B — red cells form the packed column at the bottom. C — plasma is the fluid column above the buffy coat. D — bacteria aren't what this layer represents.

Source: eClinpath (Cornell University), "Hematocrit — Method of measurement"

Question 25 of 50 · Anesthesia

A diabetic dog that receives insulin is scheduled for anesthesia. What does the 2020 AAHA guideline suggest for the day of the procedure?
Check answer for question 25

Answer: D. Feed half the normal meal and give half the insulin dose 2–4 hours before, monitor blood glucose, and schedule the dog as the first case of the day

AAHA's fasting recommendations for diabetic patients call for half a meal and half the insulin dose 2–4 hours before the procedure, blood-glucose monitoring before, during, and after anesthesia, and scheduling as the first case. The veterinarian must individualize the feeding and insulin instructions for the patient; the figure is guidance, not an exclusive protocol.

Why not the others: A — a full insulin dose without food risks hypoglycemia. B and C — neither matches AAHA's recommendation.

Source: 2020 AAHA Anesthesia and Monitoring Guidelines, “Fasting and Treatment Recommendations for Dogs and Cats Prior to Anesthesia,” diabetic row and individual-patient guidance, PDF p.1; Vetsulin manufacturer label (rev. Jul 2026), DailyMed, Owner Information Sheet: possible side effects and causes of hypoglycemia

Question 26 of 50 · Animal Care and Nursing

A dog's Schirmer tear test reads 9 mm/min. How should this be interpreted?
Check answer for question 26

Answer: B. Below normal — consistent with keratoconjunctivitis sicca (dry eye)

The American College of Veterinary Ophthalmologists describes more than 15 mm/min as normal in dogs and less than 15 mm/min as suggestive of, or diagnostic for, keratoconjunctivitis sicca (KCS).

Why not the others: A — 9 mm/min is below the normal threshold. C — a low reading means reduced, not excessive, tear production. D — the test has a clear normal cut-off.

Source: ACVO, “Dry Eye Disease or Keratoconjunctivitis Sicca (KCS)” (22 Sep 2020), “How is KCS diagnosed?”

Question 27 of 50 · Surgical Nursing

For an uncomplicated surgical site with intact skin, the selected gauze-and-antiseptic preparation protocol uses concentric circles. In which direction should preparation proceed?
Check answer for question 27

Answer: C. Starting at the planned incision site and working outward toward the periphery

In this concentric-circle gauze protocol, surgical-site preparation starts at the anticipated incision site and works outward toward the periphery. Discard the used sponge at the edge and use a fresh sponge for the next pass. Application technique and contact time must follow the selected product and protocol.

Why not the others: A — moving inward carries contamination toward the incision. B — back-and-forth strokes are not the selected concentric-circle technique; some other products do specify them. D — use the specified application pattern and contact time, not an arbitrary direction or amount.

Source: University of Illinois Animal Care and Use, “Preparation of the Surgical Site” (rev. 6 Mar 2021), Step 4 “Final Skin Preparation”; Veterinary Clinical Skills Compendium, “Patient prep,” “Scrub from center to perimeter” and How to instructions; ChloraPrep One-Step prescribing/product label, DailyMed, “Directions” (updated 23 Oct 2025)

Question 28 of 50 · Pharmacy and Pharmacology · Calculation

A drug is supplied at 0.5 mg/mL. The order is 5 mcg/kg for a 20-kg dog. How many mL should be drawn up?
Check answer for question 28

Answer: A. 0.2 mL

Dose = 5 mcg/kg × 20 kg = 100 mcg = 0.1 mg. Volume = 0.1 mg ÷ 0.5 mg/mL = 0.2 mL. Convert micrograms to milligrams before dividing.

Why not the others: B — treats 100 mcg as 1 mg (a tenfold error). C — treats 100 mcg as 10 mg (a hundredfold error). D — slips the decimal the other way.

Source: Worked arithmetic (1 mg = 1,000 mcg); applied mathematics is Knowledge Statement 7 in the AAVSB VTNE Content Outline

Question 29 of 50 · Dentistry

Under AVDC nomenclature, a single-rooted tooth with about 15% attachment loss is in which periodontal disease stage?
Check answer for question 29

Answer: D. PD2

AVDC stages: PD0 normal; PD1 gingivitis without attachment loss; PD2 less than 25% attachment loss; PD3 25–50%; PD4 more than 50%. Fifteen percent falls in PD2.

Why not the others: A — PD0 is a normal tooth. B — PD1 has no attachment loss. C — PD3 begins at 25%.

Source: American Veterinary Dental College, AVDC Nomenclature, “Stages of Periodontal Disease” (live reference)

Question 30 of 50 · Emergency Medicine/Critical Care

A dog is brought in after drinking antifreeze. Which crystals in the urine sediment support ethylene glycol toxicosis?
Check answer for question 30

Answer: B. Calcium oxalate

Merck notes that calcium oxalate crystals — monohydrate most commonly, and dihydrate — may appear in ethylene glycol toxicosis, as early as about 3 hours after ingestion in cats and 6 hours in dogs.

Why not the others: A, C, D — none is the crystal type associated with ethylene glycol.

Source: Merck Veterinary Manual, "Ethylene Glycol Toxicosis in Animals," Diagnosis (updated Sep 2024); Cornell eClinpath, “Crystals,” calcium oxalate dihydrate/monohydrate, struvite, cystine, and bilirubin sections

Question 31 of 50 · Animal Care and Nursing

A handler wants a cow with an established flight zone to walk forward along an alley. Relative to the cow's point of balance at the shoulder, where should the handler stand?
Check answer for question 31

Answer: C. Behind the shoulder, inside the edge of the flight zone

The point of balance is at the cow's shoulder. A handler positioned behind the shoulder moves the cow forward; one positioned in front of the shoulder moves it backward. Cattle move when a person enters their flight zone — the animal's personal space.

Why not the others: A and B — standing in front of the shoulder tends to make the cow back up, not walk forward. D — standing outside the flight zone does not apply the flight-zone pressure used to prompt this movement.

Source: Cooperative Extension Ag Safety and Health, "What Is a Cow's Flight Zone?" (May 17, 2019)

Question 32 of 50 · Anesthesia

At the end of a busy day, the CO₂ absorbent in the anesthesia machine had turned purple after more than 8 hours of use. The next morning it looks white again. What should the technician do?
Check answer for question 32

Answer: A. Replace it — the color can revert to white even when the absorbent is exhausted

Standard soda lime turns purple as it is exhausted and can revert to white after resting, so the color alone can't be trusted the next day. AAHA recommends changing absorbent after 8 hours of use.

Why not the others: B — color reversion makes "white" misleading. C — exhausted absorbent lets CO₂ be rebreathed; a falling ETCO₂ is not a replacement trigger. D — shaking doesn't restore spent absorbent.

Source: 2020 AAHA Anesthesia and Monitoring Guidelines, Step 2a, PDF p.7 (8-hour interval), and Step 2b, PDF p.8 (CO₂ absorption in rebreathing circuits); Midmark, “A Practical Guide for Soda Lime Replacement in Veterinary Anesthesia” (15 Jul 2025), “How New Color Indicators Can Help”

Question 33 of 50 · Laboratory Procedures

Colorless, three-dimensional, prism-shaped crystals that look like "coffin lids" are seen in a urine sediment. What are they?
Check answer for question 33

Answer: B. Struvite (magnesium ammonium phosphate)

eClinpath describes struvite crystals as colorless, three-dimensional prisms often likened to coffin lids.

Why not the others: A, C, D — each has a different characteristic appearance on eClinpath's crystal reference; the coffin-lid prism is struvite.

Source: eClinpath, "Crystals — Struvite"

Question 34 of 50 · Surgical Nursing

Which part of a sterile surgical gown is considered sterile once it is on?
Check answer for question 34

Answer: D. The front from chest to the level of the sterile field, and the sleeves from about 2 inches above the elbow to, but not including, the cuff

AORN's cited sterile-field recommendations limit the sterile area of a gown to the front from chest to the level of the sterile field, and the sleeves from 2 inches above the elbow to, but not including, the cuff. The cuffs should remain covered by the sterile gloves.

Why not the others: A and C — the back can't be watched and isn't considered sterile. B — below the level of the sterile field is not considered sterile.

Source: AORN, Recommended Practices for Maintaining a Sterile Field, 2012 compilation (last revised Nov 2005), Recommendation I, items 5–6, printed p.87 (one-page preview)

Question 35 of 50 · Pharmacy and Pharmacology

A healthy dog is premedicated with acepromazine. Which cardiovascular effect should the technician watch for?
Check answer for question 35

Answer: A. Hypotension from vasodilation

Merck explains that acepromazine's alpha-1 adrenergic blocking effect on vascular smooth muscle causes vasodilation, and hypotension can occur.

Why not the others: B and D — vasodilation lowers, not raises, blood pressure. C — not the effect Merck highlights for acepromazine.

Source: Merck Veterinary Manual, "Tranquilizers, Sedatives, and Analgesics," section "Acepromazine as a Tranquilizer or Sedative for Animals" (updated Aug 2025)

Question 36 of 50 · Animal Care and Nursing

A dog has semidry (tacky) mucous membranes, normal skin turgor, and normal eye moisture. On Merck's dehydration grading scale, this fits about:
Check answer for question 36

Answer: C. 4–5%

Merck's lowest dehydration grade, 4–5%, is defined by semidry mucous membranes with normal skin turgor and normal eye moisture. Higher grades require additional findings. Merck also stresses that these estimates are subjective.

Why not the others: A — on this scale, these findings fit the 4–5% estimate rather than 0%. B and D — higher grades need more abnormal findings than tacky membranes alone.

Source: Merck Veterinary Manual, "The Fluid Resuscitation Plan in Animals," section "Determination of the Fluid Deficit" (reviewed Nov 2025)

Question 37 of 50 · Diagnostic Imaging

What happens to a film-screen radiograph when kVp is increased and other factors are adjusted to keep overall film density similar?
Check answer for question 37

Answer: B. Beam penetration rises and contrast falls (a longer scale of contrast)

Higher kVp gives the beam more penetrating power and narrows the differences in attenuation between tissues, so contrast drops and the image shows more shades of gray (long-scale contrast).

Why not the others: A — reverses the relationship. C — kVp is the main control of contrast. D — penetration increases, not decreases.

Source: Merck Veterinary Manual, "Radiography of Animals," sections "Equipment in Radiography of Animals" and "Filmless (Digital) Radiography of Animals" (updated Apr 2025)

Question 38 of 50 · Anesthesia

When should an endotracheal tube be removed from a brachycephalic dog recovering from anesthesia?
Check answer for question 38

Answer: D. Once the dog can protect its airway by swallowing vigorously, with normal breathing and SpO₂

AAHA directs extubation when respiratory rate and SpO₂ are normal and the patient can adequately protect its airway by swallowing vigorously. VASG stresses waiting for a clear swallow, especially in brachycephalic breeds.

Why not the others: A, B, C — none of these signs alone establishes that the dog can protect its airway, which is the concern in brachycephalic patients.

Source: 2020 AAHA Anesthesia and Monitoring Guidelines, Step 4e "Recovery from Anesthesia" (PDF p.20); VASG, "Recovery," item 2

Question 39 of 50 · Communication and Veterinary Professional Support Services

A client was bitten by a dog of unknown vaccination status and asks what to do first. What is the appropriate first-aid advice?
Check answer for question 39

Answer: A. Wash the wound thoroughly with soap and water right away, then seek medical care

CDC advises washing bites or scratches immediately with soap and water after a possible rabies exposure, then urgently seeking medical attention. A physician decides whether post-exposure treatment is needed; the technician's role is first aid and referral, not medical advice.

Why not the others: B and D — delaying medical evaluation after a possible rabies exposure is the dangerous choice. C — skipping washing removes the immediate first-aid step.

Source: CDC, "About Rabies" (Jan 27, 2026)

Question 40 of 50 · Surgical Nursing

Which scissors are designed for dissecting delicate tissue?
Check answer for question 40

Answer: C. Metzenbaum scissors

Metzenbaum scissors are fine scissors for delicate tissue and blunt dissection.

Why not the others: A — Mayo scissors are for heavier tissue such as fascia. B and D — made for bandage material and wire, not for tissue dissection.

Source: University of Saskatchewan WCVM, VSAC 205 Lab 1 "Scissors"; WVS Academy, "Instruments," section "Scissors" — "Non-tissue scissors" and "Tissue scissors"

Question 41 of 50 · Pharmacy and Pharmacology · U.S. federal rule

Under U.S. federal rules, which drug is prohibited from extralabel use in food-producing animals?
Check answer for question 41

Answer: D. Chloramphenicol

21 CFR 530.41 lists drugs prohibited for extralabel use in food-producing animals; chloramphenicol heads the list (others include clenbuterol, diethylstilbestrol, nitroimidazoles, and furazolidone).

Why not the others: A, B, C — none appears on the 530.41 prohibited list.

Source: 21 CFR 530.41(a)(1) (eCFR, displayed content up to date as of 15 Oct 2026; checked 17 Oct 2026 UTC)

Question 42 of 50 · Animal Care and Nursing

A severely underweight cat that has not eaten for days starts nutritional support. Which electrolyte abnormality is the hallmark of refeeding syndrome?
Check answer for question 42

Answer: B. Hypophosphatemia

MSPCA-Angell's critical-care team calls hypophosphatemia the hallmark sign of refeeding syndrome, often alongside hypokalemia and hypomagnesemia.

Why not the others: A — phosphorus falls, not rises. C and D — not the defining changes of refeeding syndrome.

Source: MSPCA-Angell, "Refeeding Syndrome" (Sumner, DACVECC; Feb 2026)

Question 43 of 50 · Dentistry

Using the bisecting angle technique for an intraoral dental radiograph, the x-ray beam is aimed:
Check answer for question 43

Answer: C. Perpendicular to an imaginary line that bisects the angle between the tooth's long axis and the sensor

When the sensor can't sit parallel to the tooth, the angle between the root and the sensor is bisected, and the beam is aimed perpendicular to that bisecting line. In dogs and cats, the parallel technique works only for the caudal mandibular premolars and molars.

Why not the others: A and B — each makes the beam perpendicular to one side of the angle instead of the line that bisects it. D — a beam running parallel to the sensor wouldn't strike it to make an image.

Source: Niemiec B, "Practical Dentistry: Techniques for Intraoral Radiology," Today's Veterinary Practice (14 Aug 2015), "Step 3. Positioning the Tube Head"

Question 44 of 50 · Laboratory Procedures

Where on a blood smear should the leukocyte differential count be performed?
Check answer for question 44

Answer: A. In the monolayer, just behind the feathered edge

eClinpath identifies the monolayer — about one 10× field behind the feathered edge, where cells lie side by side without overlapping — as the area for differential counts.

Why not the others: B — in the thick body of the smear, cells crowd together and white cells look shrunken, so they are hard to identify. C — at the feathered edge, white cells are smudged, red cells flatten, and large cells collect there. D — location changes what you see, so it changes the count.

Source: eClinpath, "Blood Smear Examination — Components of a blood smear: Monolayer"

Question 45 of 50 · Pain Management/Analgesia

What does the 2022 AAHA Pain Management Guidelines mean by a multimodal approach?
Check answer for question 45

Answer: D. Combining drugs or methods that act on pain in different ways, so no single drug carries the whole plan

AAHA's guiding principles call for a multimodal approach that interrupts pain pathways using multiple differing approaches, so the plan doesn't rely on any single drug.

Why not the others: A and B — single-drug strategies are what multimodal plans avoid. C — waiting for obvious pain isn't a multimodal strategy.

Source: Gruen ME et al., 2022 AAHA Pain Management Guidelines for Dogs and Cats, JAAHA 58(2), Introduction (printed p.56; PDF p.2) and "Acute Pain of Known Cause (e.g., Perioperative or Diagnosed, Known Trauma)" (printed p.66; PDF p.12)

Question 46 of 50 · Anesthesia

According to the 2020 AAHA anesthesia guidelines, below what body temperature is an anesthetized patient hypothermic, and what should the team do?
Check answer for question 46

Answer: B. Below 98 °F (36.7 °C); monitor temperature and start heat support early

AAHA defines hypothermia as below 98 °F (36.7 °C), notes it causes multiple complications, and calls temperature monitoring critical, with heat supplementation starting early.

Why not the others: A — AAHA says to start heat support early, not after recovery. C — sets the threshold too low. D — AAHA calls temperature monitoring critical.

Source: 2020 AAHA Anesthesia and Monitoring Guidelines, Step 4d (PDF pp.18–19) and Step 4e (PDF p.20); early temperature support (PDF p.13)

Question 47 of 50 · Surgical Nursing

Which suture material is absorbable and monofilament?
Check answer for question 47

Answer: C. Polydioxanone (PDS)

Polydioxanone is an absorbable monofilament suture; for its PDS II product, Ethicon lists absorption at about 182–238 days.

Why not the others: A — nonabsorbable; nylon can be monofilament or braided. D — a nonabsorbable monofilament. B — nonabsorbable and braided.

Source: Merck Manual Professional, "Suture Materials" table; Ethicon, PDS II product page; Ethicon, NUROLON Nylon technical data, material and structure fields

Question 48 of 50 · Pharmacy and Pharmacology · Calculation

You need 80 mL of a 3% (w/v) solution and have a 12% (w/v) stock solution. How much stock solution do you measure before adding diluent to reach 80 mL?
Check answer for question 48

Answer: A. 20 mL

Use C₁V₁ = C₂V₂: 12% × V₁ = 3% × 80 mL, so V₁ = (3 × 80) ÷ 12 = 20 mL of stock. Then add diluent until the total volume is 80 mL (about 60 mL of diluent).

Why not the others: B — 60 mL is roughly the diluent you add, not the stock you measure. C — inverts the ratio (12 × 80 ÷ 3). D — 2.4 g is the amount of solute in the finished solution (3 g per 100 mL × 80 mL), not a volume of stock.

Source: Worked arithmetic (C₁V₁ = C₂V₂; % w/v = grams per 100 mL); applied mathematics is Knowledge Statement 7 in the AAVSB VTNE Content Outline

Question 49 of 50 · Emergency Medicine/Critical Care

An 8-year-old Great Dane is restless, drooling, retching without bringing anything up, and its abdomen is enlarging. What should the technician suspect and do?
Check answer for question 49

Answer: D. Gastric dilatation-volvulus (GDV); alert the veterinarian immediately

Merck lists nonproductive retching, hypersalivation, restlessness, and acute or progressive abdominal distension as the clinical findings of GDV, which occurs mainly in large and giant deep-chested breeds. It's an emergency.

Why not the others: A, B, C — none fits this classic presentation, and each delays emergency care.

Source: MSD Veterinary Manual (Merck), "Gastric Dilation and Volvulus in Small Animals," Epidemiology and Clinical Findings (Aug 2025)

Question 50 of 50 · Animal Care and Nursing

According to the University of Guelph protocol, a nasoesophageal feeding tube's final insertion length is premeasured from the tip of a dog's nose to which landmark?
Check answer for question 50

Answer: B. The 8th intercostal space

The University of Guelph protocol measures the nasoesophageal tube's final insertion length from the nose tip to the 8th intercostal space. Premeasurement does not confirm correct placement. The chapter requires confirmation before delivering fluids through the tube and identifies radiography as the safest way to document proper placement.

Why not the others: A — the 13th rib is the landmark for a nasogastric tube, which ends in the stomach. C — the medial canthus is a nearby landmark on the head, not the tube's end point. D — the thoracic inlet is the first landmark used during placement, not the final insertion-length landmark.

Source: University of Guelph, VETM 4540 "Nasoesophageal and Nasogastric Tube Placement," sections "Landmarks" and "Procedure"

Score your practice test

Give yourself one point for each question you answered correctly on your first try, before reading the explanation. Your total is a result on these 50 questions — not a VTNE score and not a prediction of passing. The real VTNE reports a scaled score from 200 to 800, 425 passes, and AAVSB does not report number or percent correct, so a percentage on this set cannot be converted into an official pass or fail. (AAVSB Candidate Handbook, "Scoring")

Want to practice at exam pace? The VTNE allows 3 hours for 170 questions — about 64 seconds each — so give yourself about 53 minutes for these 50.

Answer key

QuestionAnswerDomain
1C. 1–2 secondsAnimal Care and Nursing
2A. A U-40 insulin syringePharmacy and Pharmacology
3D. 30 minutesSurgical Nursing
4B. 800–1,600 mL/minAnesthesia
5A. Centrifugal flotation using a solution with a specific gravity of 1.18–1.27Laboratory Procedures
6C. Support the hind legs and tuck the hindquarters into the crook of the armAnimal Care and Nursing
7B. Deeper than the usual 1–3 mm reference range for dogs — chart it and report itDentistry
8D. Blood type the cat (A/B/AB); a major crossmatch is also strongly recommendedEmergency Medicine/Critical Care
9A. NaloxonePain Management/Analgesia
10C. Enters the ventral surface and exits the dorsal surfaceDiagnostic Imaging
11B. A sodium hypochlorite product labeled effective against canine parvovirus, used at its labeled dilution and full wet contact timeAnimal Care and Nursing
12A. Loperamide should be avoided in dogs that carry the mutation.Pharmacy and Pharmacology
13D. Opened (unlocked) so steam can contact all surfacesSurgical Nursing
14C. Pressure buildup in the circuit and lungs, causing pulmonary barotraumaAnesthesia
15B. Rigor mortisCommunication and Veterinary Professional Support Services
16A. About 394 kcal/dayAnimal Care and Nursing
17D. Adult female wormsLaboratory Procedures
18B. Clean-contaminatedSurgical Nursing
19C. The tube is in the esophagusAnesthesia
20A. Retinal toxicity, including blindnessPharmacy and Pharmacology
21D. A pulse deficit — suggestive of an arrhythmia; report it, since an ECG is indicatedAnimal Care and Nursing
22B. Acute renal failure and death with repeated usePain Management/Analgesia
23C. At collar level, outside the lead apron and thyroid shieldDiagnostic Imaging
24A. White blood cells and plateletsLaboratory Procedures
25D. Feed half the normal meal and give half the insulin dose 2–4 hours before, monitor blood glucose, and schedule the dog as the first case of the dayAnesthesia
26B. Below normal — consistent with keratoconjunctivitis sicca (dry eye)Animal Care and Nursing
27C. Starting at the planned incision site and working outward toward the peripherySurgical Nursing
28A. 0.2 mLPharmacy and Pharmacology
29D. PD2Dentistry
30B. Calcium oxalateEmergency Medicine/Critical Care
31C. Behind the shoulder, inside the edge of the flight zoneAnimal Care and Nursing
32A. Replace it — the color can revert to white even when the absorbent is exhaustedAnesthesia
33B. Struvite (magnesium ammonium phosphate)Laboratory Procedures
34D. The front from chest to the level of the sterile field, and the sleeves from about 2 inches above the elbow to, but not including, the cuffSurgical Nursing
35A. Hypotension from vasodilationPharmacy and Pharmacology
36C. 4–5%Animal Care and Nursing
37B. Beam penetration rises and contrast falls (a longer scale of contrast)Diagnostic Imaging
38D. Once the dog can protect its airway by swallowing vigorously, with normal breathing and SpO₂Anesthesia
39A. Wash the wound thoroughly with soap and water right away, then seek medical careCommunication and Veterinary Professional Support Services
40C. Metzenbaum scissorsSurgical Nursing
41D. ChloramphenicolPharmacy and Pharmacology
42B. HypophosphatemiaAnimal Care and Nursing
43C. Perpendicular to an imaginary line that bisects the angle between the tooth's long axis and the sensorDentistry
44A. In the monolayer, just behind the feathered edgeLaboratory Procedures
45D. Combining drugs or methods that act on pain in different ways, so no single drug carries the whole planPain Management/Analgesia
46B. Below 98 °F (36.7 °C); monitor temperature and start heat support earlyAnesthesia
47C. Polydioxanone (PDS)Surgical Nursing
48A. 20 mLPharmacy and Pharmacology
49D. Gastric dilatation-volvulus (GDV); alert the veterinarian immediatelyEmergency Medicine/Critical Care
50B. The 8th intercostal spaceAnimal Care and Nursing

Find your weak spots

Tally your correct answers by domain using the question numbers in the coverage table below. With only 2 to 10 questions per domain, a domain result tells you where to look first. It isn't a precise measure of what you know.

Turn each miss into a review task

For every question you missed or skipped, write down the specific idea you need to be able to explain. Reread that question's source, then answer the question again with the explanation covered. Two examples:

QuestionWhat went wrongWhat to practiceNext step
4Multiplied by the rebreathing rate (20–40 mL/kg/min) instead of the non-rebreathing rateMatch the circuit to its oxygen-flow range, then multiply by body weightRedo question 4 from scratch, writing units on every line
12Assumed every ivermectin product is unsafe for dogs with the MDR1 mutationSeparate label-dose heartworm prevention from other ivermectin uses; know loperamide is the drug to avoidReread Cornell's "Drug sensitivity: MDR1" page, then retry question 12
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What this practice test covers

The 50 questions follow the official VTNE Content Outline (approved March 2023), with about one-third of each domain's scored items, rounded. The outline sets the topics; the linked teaching sources support the explanations, and the calculation items show their arithmetic.

DomainScored items on the VTNEQuestions in this testTopics here
Pharmacy and Pharmacology207 (Q2, Q12, Q20, Q28, Q35, Q41, Q48)U-40 insulin syringes, MDR1 drug sensitivity, enrofloxacin in cats, microgram dose math, acepromazine, extralabel-use rules, dilution math
Surgical Nursing207 (Q3, Q13, Q18, Q27, Q34, Q40, Q47)Steam cycle times, hinged instruments, wound classification, skin prep, sterile gown, scissors, suture
Dentistry103 (Q7, Q29, Q43)Probing depth, periodontal staging, bisecting-angle radiography
Laboratory Procedures145 (Q5, Q17, Q24, Q33, Q44)Fecal flotation, heartworm antigen, buffy coat, struvite crystals, smear monolayer
Animal Care and Nursing3010 (Q1, Q6, Q11, Q16, Q21, Q26, Q31, Q36, Q42, Q50)Capillary refill, rabbit handling, parvovirus disinfection, energy (RER) math, pulse deficit, Schirmer tear test, cattle handling, dehydration grading, refeeding syndrome, nasoesophageal tubes
Diagnostic Imaging93 (Q10, Q23, Q37)Projection names, dosimeter placement, kVp and contrast
Anesthesia207 (Q4, Q14, Q19, Q25, Q32, Q38, Q46)Non-rebreathing oxygen flow math, APL valve, capnography and tube placement, diabetic patients, CO₂ absorbent, extubation, hypothermia
Emergency Medicine/Critical Care103 (Q8, Q30, Q49)Feline transfusion, ethylene glycol, GDV
Pain Management/Analgesia103 (Q9, Q22, Q45)Opioid reversal, meloxicam in cats, multimodal analgesia
Communication and Veterinary Professional Support Services72 (Q15, Q39)Confirming death after euthanasia, bite first-aid advice
Total15050Blank

The VTNE's scored item counts show the official domain weighting. They don't tell you how hard a domain is or how long you need to study it. This set covers dogs, cats, rabbits and cattle, but it can't sample every task or species on the outline.

How this compares with the real VTNE

Row labelReal VTNEThis free practice test
Questions170: 150 scored + 20 unscored pilot questions you can't tell apart50, all counted
Time3 hoursUntimed, or about 53 minutes at exam pace
FormatFour options, one correct or best answer; no penalty for guessingSame format
ScoringScaled 200–800; 425 passesYour own count of correct answers
CalculatorOn-screen calculator providedUse your own
Cost$375 application feeFree, no account

Sources: AAVSB Candidate Information Handbook ("Content," p.7; "Fees," p.9; "Taking the Exam by Computer," p.20; calculator policy, p.22; "Scoring," p.29); AAVSB, Schedule and Take the VTNE.

On the real exam you can skip questions, change answers and mark questions to come back to. Handheld calculators aren't allowed unless you have an approved accommodation.

Passing the VTNE doesn't license you by itself. Your state or provincial board issues the credential, and AAVSB sends it your score. Exam windows, application cutoffs and retake rules are in the current candidate handbook.

Free VTNE resources

Sources and how we checked

Every question's teaching source is linked under its explanation. Exam facts on this page come from:

Last verified: October 17, 2026. We checked the exam format, scoring and content outline against AAVSB's current handbook and website, and we checked each answer against the source linked with it.

How these questions were made: The Castleport Test Prep Editorial Team wrote these questions with AI assistance. Each question is original and is not a real VTNE item; AAVSB prohibits copying or sharing actual exam questions. Our checking was a source check — no credentialed veterinary professional has reviewed this set. If you spot an error, please tell us.

By Castleport Test Prep Editorial Team

Castleport Test Prep is an independent exam prep publisher. We are not affiliated with, endorsed by, or approved by the American Association of Veterinary State Boards (AAVSB) or PSI. Exam and credential names identify their subjects, and trademarks belong to their respective owners. These practice questions are unofficial, and this page does not guarantee an exam result or a credential.

Free VTNE Practice Test: 50 Questions With Answers Explained